US 11,692,939 B2
Spectral edge detection
Jeremy Ryan Cooper, North Bend, WA (US)
Assigned to RareCyte, Inc., Seattle, WA (US)
Filed by RareCyte, Inc., Seattle, WA (US)
Filed on Jul. 21, 2020, as Appl. No. 16/934,297.
Application 16/934,297 is a continuation of application No. 16/503,118, filed on Jul. 3, 2019, granted, now 10,753,875.
Application 16/503,118 is a continuation in part of application No. 16/264,490, filed on Jan. 31, 2019, granted, now 10,345,237, issued on Jul. 9, 2019.
Prior Publication US 2020/0348233 A1, Nov. 5, 2020
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6458 (2013.01) [G01N 21/6428 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6439 (2013.01)] 32 Claims
 
1. A method of analyzing a sample, the sample comprising a first component, the first component having a first autofluorescent emission spectrum, the first autofluorescent emission spectrum having a peak emission intensity at a peak intensity wavelength, a leading spectral edge with emission intensities less than the peak intensity at wavelengths less than the peak intensity wavelength, and a trailing spectral edge with emission intensities less than the peak intensity at wavelengths greater than the peak intensity wavelength, the method comprising:
acquiring, by stimulating the sample with excitation light, a first raw image of the sample at a first emission wavelength of the first autofluorescent emission spectrum of the first component, wherein the first emission wavelength is in the leading spectral edge or in the trailing spectral edge of the first autofluorescent emission spectrum, and wherein the first raw image comprises a first total signal comprising a first autofluorescent signal at the first emission wavelength;
acquiring, by stimulating the sample with excitation light, a second raw image of the sample at a second emission wavelength of the first autofluorescent emission spectrum of the first component, wherein the second raw image comprises a second total signal comprising a second autofluorescent signal at the second emission wavelength; and
outputting a final image of the first component based on the first and second raw images, and
wherein the first component comprises a non-detection moiety.