| CPC G01N 21/6408 (2013.01) [G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01); G01N 2201/127 (2013.01)] | 8 Claims |
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1. A spectral calibration apparatus for calculating a color conversion matrix used in color conversion processing, comprising:
a spectral signal acquisition unit that acquires a spectral signal of fluorescence detected over time;
an initial calculation unit the calculates initial values of a color conversion matrix and initial values of a temporal activation of fluorescence of the spectral signal by
removing a spike signal from the spectral signal, to obtain a spike-less signal,
removing a baseline from the spike-less signal, to obtain a modified signal,
normalizing an intensity between wavelength channels based on the modified signal, to obtain a normalized signal,
calculating the initial values of the color conversion matrix using the normalized signal, and
calculating the initial values of the temporal activation of fluorescence using the initial values of the color conversion matrix;
a candidate calculation unit that calculates a candidate of the color conversion matrix for each value of a parameter using the initial values of the color conversion matrix and the initial values of the temporal activation of fluorescence, which depends on a frequency at which fluorescence peaks of fluorescent dyes appear at the same time, based on the spectral signal; and
a selection unit that selects a color conversion matrix based on an evaluation value calculated for each candidate.
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