| CPC C12Q 1/6869 (2013.01) [C12Q 1/6876 (2013.01); G01N 21/6486 (2013.01); B01L 2200/10 (2013.01)] | 19 Claims |
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1. A method for sequencing a nucleic acid using an immersion reaction protocol, the immersion reaction protocol comprising the following steps:
a) providing a first batch of solid supports having nucleic acid molecules immobilized thereon,
b) immersing the first batch of solid supports in one or more reaction solutions to generate a signal representing a nucleotide of the nucleic acid molecule on the solid supports, wherein each of the one or more reaction solutions is placed in a separate reaction container,
wherein, after removing the first batch of solid supports from each of the reaction solutions, washing the first batch of solid supports to remove the reaction solution remaining on the solid supports,
c) detecting the signal on the first batch of solid supports,
d) immersing the first batch of solid supports in a regeneration solution placed in another reaction container to eliminate the signal on the solid supports,
e) removing the first batch of solid supports from the regeneration solution, and washing the first batch of solid supports to remove the regeneration solution remaining on the solid supports, and
f) optionally, repeating the steps b) to e) or the steps b) to c), and
g) determining the nucleotide sequence of the nucleic acid molecule based on the detected signals.
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