US 11,692,219 B2
Construction of next generation sequencing (NGS) libraries using competitive strand displacement
Zachary Zwirko, Redwood City, CA (US); Yu Zheng, Redwood City, CA (US); Mirna Jarosz, Mountain View, CA (US); Caifu Chen, Palo Alto, CA (US); and Joseph Walder, Chicago, IL (US)
Assigned to INTEGRATED DNA TECHNOLOGIES, INC., Coralville, IA (US)
Filed by INTEGRATED DNA TECHNOLOGIES, INC., Coralville, IA (US)
Filed on Jan. 5, 2021, as Appl. No. 17/141,636.
Application 17/141,636 is a continuation of application No. 16/138,845, filed on Sep. 21, 2018, granted, now 10,894,979.
Application 16/138,845 is a continuation in part of application No. 15/880,762, filed on Jan. 26, 2018, granted, now 10,683,542, issued on Jun. 16, 2020.
Application 16/138,845 is a continuation in part of application No. PCT/US2018/015391, filed on Jan. 26, 2018.
Claims priority of provisional application 62/451,267, filed on Jan. 27, 2017.
Claims priority of provisional application 62/562,739, filed on Sep. 25, 2017.
Prior Publication US 2021/0147926 A1, May 20, 2021
Int. Cl. C12Q 1/6855 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01); C40B 80/00 (2006.01); C40B 40/06 (2006.01); C40B 20/04 (2006.01)
CPC C12Q 1/6855 (2013.01) [C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C40B 40/06 (2013.01); C12Q 1/6869 (2013.01); C40B 20/04 (2013.01); C40B 80/00 (2013.01)] 7 Claims
 
1. A sequencing adapter comprising:
a first DNA strand and a second DNA strand;
wherein the first DNA strand comprises a nucleotide sequence of SEQ ID NO: 18 that includes a 5′ phosphate and a blocking group at its 3′ end;
wherein the second DNA strand comprises a nucleotide sequence of SEQ ID NO: 34 that includes a dideoxy nucleotide (ddN) at its 3′ end; and
wherein the second DNA strand is at least partially complementary to the first DNA strand.