CPC C12Q 1/6855 (2013.01) [C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C40B 40/06 (2013.01); C12Q 1/6869 (2013.01); C40B 20/04 (2013.01); C40B 80/00 (2013.01)] | 7 Claims |
1. A sequencing adapter comprising:
a first DNA strand and a second DNA strand;
wherein the first DNA strand comprises a nucleotide sequence of SEQ ID NO: 18 that includes a 5′ phosphate and a blocking group at its 3′ end;
wherein the second DNA strand comprises a nucleotide sequence of SEQ ID NO: 34 that includes a dideoxy nucleotide (ddN) at its 3′ end; and
wherein the second DNA strand is at least partially complementary to the first DNA strand.
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