CPC C12Q 1/6806 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12Q 1/6874 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] | 14 Claims |
1. A composition comprising a mixture of DNA targeted sequences for depletion and DNA sequences of interest, wherein the DNA targeted sequences for depletion and the DNA sequences of interest are adapter-ligated, wherein the adapters are ligated to both the 5′ and 3′ ends of both the target sequences for depletion and the sequences of interest, and wherein the DNA targeted sequences for depletion are complexed to a gRNA-CRISPR/Cas system protein complex and wherein the DNA sequences of interest are in the range of about 0.1% to about 50% of the DNA in the composition, wherein the CRISPR/Cas system protein is Cas9, Cpf1, Cas3, Cas8a-c, Cas10, Csel, Csyl, Csn2, Cas4, Csm2, or CmS.
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