	US 11,692,208 B2
	Production of chemicals from renewable sources
Harshal Akshay Chokhawala, Castro Valley, CA (US); Jonathan Kuchenreuther, Fremont, CA (US); Jorge Alonso-Gutiérrez, San Francisco, CA (US); and Yi-Shu Tai, Alameda, CA (US)
Assigned to ZYMOCHEM, INC., San Leandro, CA (US)
Filed by ZYMOCHEM, INC., San Leandro, CA (US)
Filed on Sep. 16, 2022, as Appl. No. 17/947,048.
Application 17/947,048 is a continuation of application No. 17/605,389, previously published as PCT/US2020/029981, filed on Apr. 25, 2020.
Claims priority of provisional application 62/868,824, filed on Jun. 28, 2019.
Claims priority of provisional application 62/838,793, filed on Apr. 25, 2019.
Prior Publication US 2023/0109883 A1, Apr. 13, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/20 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12P 7/40 (2006.01); C12P 7/625 (2022.01); C12P 7/62 (2022.01)

CPC C12P 7/625 (2013.01) [C12N 1/20 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12P 7/40 (2013.01); C12P 7/62 (2013.01); C12Y 106/05005 (2013.01); C12Y 401/01004 (2013.01); C12Y 401/02045 (2013.01); C12Y 402/0103 (2013.01); C12Y 402/01034 (2013.01); C12N 2800/101 (2013.01)]

14 Claims

1. A method, comprising:

contacting pyruvate and HO—CH₂—CH₂—CHO (3-hydroxy propanal) with a polypeptide having the sequence of SEQ ID NO: 27 so that HO—CH₂—CH₂—CH═CH—C(O)—COOH (6-hydroxy-2-oxo-3-hexenoic acid) or a salt thereof is produced.