US 11,692,191 B2
Method for separating, capturing, analyzing and retrieving cells and cell products by using microstructure
Sung Hoon Kwon, Seoul (KR); Jun Hoi Kim, Seoul (KR); Seo Hee Chang, Suwon-si (KR); and Ok Ju Kim, Seoul (KR)
Assigned to SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
Appl. No. 16/612,880
Filed by SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
PCT Filed May 14, 2018, PCT No. PCT/KR2018/005507
§ 371(c)(1), (2) Date Nov. 12, 2019,
PCT Pub. No. WO2018/208135, PCT Pub. Date Nov. 15, 2018.
Claims priority of application No. 10-2017-0059325 (KR), filed on May 12, 2017.
Prior Publication US 2020/0199583 A1, Jun. 25, 2020
Int. Cl. C12N 15/10 (2006.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); C40B 40/00 (2006.01); C40B 60/12 (2006.01); G01N 33/543 (2006.01)
CPC C12N 15/1093 (2013.01) [B01J 19/0046 (2013.01); B01L 3/5027 (2013.01); C40B 40/00 (2013.01); C40B 60/12 (2013.01); G01N 33/54306 (2013.01); G01N 33/54386 (2013.01); B01J 2219/00644 (2013.01); B01J 2219/00743 (2013.01)] 21 Claims
 
1. A biological sample analysis chip comprising:
a microstructure-based analysis chip;
a substrate-based analysis chip; and
an array of microreaction chambers defined by the microstructure-based analysis chip and the substrate-based analysis chip,
wherein at least one of the microstructure-based analysis chip and the substrate-based analysis chip is configured to capture library-carrying cells and/or library-carrying cell products formed by biochemical reactions of the cells,
the microstructure-based analysis chip and the substrate-based analysis chip are detachable from each other and separately analyzable, and
the biological sample analysis chip further comprises an energy-absorbing layer, wherein the energy-absorbing layer is configured such that target samples located on sites of the energy-absorbing layer to which energy is applied; are separated from the microstructure-based analysis chip or the substrate-based analysis chip upon the energy application.