US 11,692,185 B2
Angiogenin expression in plants
German Spangenberg, Bundoora (AU); Aidyn Mouradov, Mill Park (AU); Jianghui Wang, Bundoora (AU); Benjamin Graeme Cocks, View Bank (AU); Matthew Knight, Airport West (AU); and Matthew McDonagh, Williamstown (AU)
Assigned to Agriculture Victoria Services PTY LTD, Bundoora (AU)
Filed by Agriculture Victoria Services PTY LTD, Bundoora (AU)
Filed on Mar. 16, 2020, as Appl. No. 16/820,051.
Application 16/820,051 is a continuation of application No. 13/510,511, abandoned, previously published as PCT/AU2010/001543, filed on Nov. 18, 2010.
Claims priority of application No. 2009905627 (AU), filed on Nov. 18, 2009.
Prior Publication US 2021/0032611 A1, Feb. 4, 2021
Int. Cl. A01H 6/54 (2018.01); A01H 6/46 (2018.01); A01H 6/20 (2018.01); A01H 6/74 (2018.01); C12N 9/22 (2006.01); C12N 15/82 (2006.01)
CPC C12N 9/22 (2013.01) [C12N 15/8257 (2013.01)] 10 Claims
 
1. A plant cell, wherein the plant cell is part of a monocotyledon or a dicotyledon plant callus, plant, seed or other plant part, said plant cell containing a nucleic acid encoding an angiogenin, wherein said nucleic acid encoding an angiogenin comprises:
(a) an N-terminal plant signal sequence that when expressed as the N-terminus of an angiogenin protein drives angiogenin accumulation in the plant cell to a sub-cellular component selected from mitochondria, chloroplast, nucleus and nucleolus, and
(b) a nucleotide sequence encoding a functional angiogenin protein, optimized for expression in a monocotyledon or a dicotyledon plant cell said monocotyledon optimized nucleotide sequence selected from the group consisting of:
(i) SEQ ID NO: 22, and
(ii) variants of SEQ ID NO: 22 having at least 98% sequence identity to the full length of SEQ ID NO: 22; and
said dicotyledon optimized nucleotide sequence selected from the group consisting of:
(iii) SEQ ID NO: 23, and
(iv) variants of SEQ ID NO: 23, having at least 98% sequence identity to the full length of SEQ ID NO: 23,
wherein said nucleic acid provides for a production of angiogenin protein at a level of between approximately 5 and 30% (weight/volume) of the total soluble protein of said plant cell,
wherein said monocotyledon is selected from the group consisting of forage grasses, sorghum, sugarcane, corn, oat, wheat, rice, and barley, and
wherein said dicotyledon is selected from the group consisting of forage legumes, soybean, lupin, peas, lentils, chickpeas, canola, vegetable brassicas, lettuce, spinach, bananas, citrus, strawberries, apples, oil palm, linseed, cottonseed, and safflower.