US 11,692,183 B2
Angiogenin expression in plants
German Spangenberg, Bundoora (AU); Aidyn Mouradov, Mill Park (AU); Jianghui Wang, Bundoora (AU); Benjamin Graeme Cocks, View Bank (AU); Matthew Knight, Airport West (AU); and Matthew McDonagh, Williamstown (AU)
Assigned to Agriculture Victoria Services PTY LTD, Bundoora (AU)
Filed by Agriculture Victoria Services PTY LTD, Attwood (AU)
Filed on May 21, 2019, as Appl. No. 16/418,407.
Application 16/418,407 is a continuation of application No. 13/510,511, abandoned, previously published as PCT/AU2010/001543, filed on Nov. 18, 2010.
Claims priority of application No. AU2009905627 (AU), filed on Nov. 18, 2009.
Prior Publication US 2019/0359960 A1, Nov. 28, 2019
Int. Cl. C12N 15/00 (2006.01); C12N 9/22 (2006.01); C12N 15/82 (2006.01)
CPC C12N 9/22 (2013.01) [C12N 15/8257 (2013.01)] 10 Claims
 
1. A plant cell, wherein the plant cell is part of a, plant, seed or other plant part, said plant cell containing a nucleic acid encoding an angiogenin, wherein said nucleic acid encoding an angiogenin comprises
(a) a C-terminal plant signal peptide that when expressed as the C-terminus of an angiogenin protein drives angiogenin accumulation in the plant cell to a sub-cellular component, and
(b) a nucleotide sequence encoding a functional angiogenin protein selected from the group consisting of:
(i) SEQ ID NO: 52, and
(ii) variants of SEQ ID NO: 52, having at least 98% sequence identity to the full length of SEQ ID NO: 52, and
wherein said plant is selected from the group consisting of a grass, a forage legume, soybean, lupin, peas, lentils, chickpeas, vegetable brassica, lettuce, spinach, bananas, citrus, strawberries, apples, oil palm, linseed, cottonseed and safflower.