	US 11,692,182 B2
	RNA-guided DNA nucleases and uses thereof
Jaishree Chittoor, Wildwood, MO (US); Ervin Nagy, Lake Saint Louis, MO (US); and Keith H. Turner, Saint Louis, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Appl. No. 15/766,662
Filed by Monsanto Technology LLC, St. Louis, MO (US)
PCT Filed Oct. 7, 2016, PCT No. PCT/US2016/056115 § 371(c)(1), (2) Date Apr. 6, 2018, PCT Pub. No. WO2017/062855, PCT Pub. Date Apr. 13, 2017.
Claims priority of provisional application 62/239,678, filed on Oct. 9, 2015.
Prior Publication US 2018/0362943 A1, Dec. 20, 2018
Int. Cl. C12N 15/82 (2006.01); C12N 9/22 (2006.01); C12N 5/04 (2006.01); C12N 15/10 (2006.01); C12N 15/90 (2006.01); C12N 15/63 (2006.01); C12N 15/11 (2006.01)

CPC C12N 9/22 (2013.01) [C12N 5/04 (2013.01); C12N 15/102 (2013.01); C12N 15/11 (2013.01); C12N 15/111 (2013.01); C12N 15/63 (2013.01); C12N 15/8213 (2013.01); C12N 15/90 (2013.01); C12N 15/902 (2013.01); C07K 2319/09 (2013.01); C07K 2319/60 (2013.01); C12N 2310/20 (2017.05); C12N 2510/00 (2013.01); C12N 2800/80 (2013.01)]

32 Claims

1. A non-naturally occurring system for sequence-specific modification of a target nucleic acid sequence, the system comprising: (a) one or more guide RNAs or a DNA molecule encoding the one or more guide RNAs; and (b) a CRISPR enzyme having the amino acid sequence of SEQ ID NO:73 or polynucleotide encoding the CRISPR enzyme, wherein the one or more guide RNAs and the CRISPR enzyme do not naturally occur together.