	US 11,692,181 B2
	Esterases and uses thereof
Isabelle Andre, Toulouse (FR); Vincent Tournier, Toulouse (FR); Alexandre Gilles, Toulouse (FR); and Sophie Duquesne, Toulouse (FR)
Assigned to CARBIOS, Saint-Beauzire (FR)
Appl. No. 17/263,574
Filed by CARBIOS, Saint-Beauzire (FR)
PCT Filed Jul. 26, 2019, PCT No. PCT/EP2019/070288 § 371(c)(1), (2) Date Jan. 27, 2021, PCT Pub. No. WO2020/021117, PCT Pub. Date Jan. 30, 2020.
Claims priority of application No. 18306019 (EP), filed on Jul. 27, 2018.
Prior Publication US 2021/0171921 A1, Jun. 10, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/18 (2006.01); C08J 11/10 (2006.01); C12N 15/52 (2006.01)

CPC C12N 9/18 (2013.01) [C08J 11/105 (2013.01); C12N 15/52 (2013.01); C08J 2367/02 (2013.01); C12Y 301/01074 (2013.01)]

22 Claims

1. An esterase which (i) comprises an amino acid sequence consisting of the amino acid sequence set forth in SEQ ID NO: 1, (ii) with one to fourteen amino acid substitutions selected from the group consisting of A17T, T27S, S48T, L82I, F90L, Y92F, G135A, A140S, N143I, S145T, A149G, S164P, V167Q, S206T, or N213P wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO: 1, and (iii) exhibits increased polyester degrading activity and/or increased thermostability compared to an esterase of SEQ ID NO: 1.