	US 11,692,178 B2
	Genetically engineered bacterium for producing L-histidine and use thereof
Xixian Xie, Tianjin (CN); Weiming Fan, Shaoxing (CN); Heyun Wu, Tianjin (CN); Wei Jiang, Shaoxing (CN); Daoguang Tian, Tianjin (CN); Yanna Chen, Shaoxing (CN); Yue Zhang, Tianjin (CN); and Jianqing Tu, Shaoxing (CN)
Assigned to ZHEJIANG ZHENYUAN BIOTECH CO., LTD., Shaoxing (CN); and Tianjin University of Science and Technology, Tianjin (CN)
Filed by ZHEJIANG ZHENYUAN BIOTECH CO., LTD., Shaoxing (CN); and Tianjin University of Science and Technology, Tianjin (CN)
Filed on Jun. 18, 2021, as Appl. No. 17/351,369.
Prior Publication US 2022/0403348 A1, Dec. 22, 2022
Int. Cl. C12N 9/10 (2006.01); C12N 9/06 (2006.01); C12P 13/24 (2006.01); C07K 14/245 (2006.01)

CPC C12N 9/1077 (2013.01) [C07K 14/245 (2013.01); C12N 9/0016 (2013.01); C12P 13/24 (2013.01); C12Y 104/01003 (2013.01); C12Y 204/02017 (2013.01)]

12 Claims

1. A genetically engineered Escherichia coli for producing L-histidine, wherein the genetically engineered Escherichia coli is constructed by taking the genome of Escherichia coli as a template and integrating a mutant encoding gene hisG* encoding the Corynebacterium glutamicum ATP phosphoribosyl transferase HisG onto the genome allowing for over-expression,

wherein the mutant encoding gene hisG* has the nucleotide sequence as shown in SEQ ID NO: 1;

over-expressing by means of increasing the number of histidine operon genes of the Escherichia coli on the genome, wherein the histidine operon genes comprise hisD, hisB, hisC, hisH, hisA, hisF and hisI;

integrating an encoding gene lysE from an arginine/lysine transport protein of the Corynebacterium glutamicum onto the genome and being over-expressed; and

integrating an encoding gene rocG of glutamate dehydrogenase of Bacillus subtilis integrated onto the genome and being over-expressed.