US 11,692,177 B2
Method for producing a chemical with synthetic microorganism encoding a monooxygenase
Elizabeth Jane Clarke, San Francisco, CA (US); Baolong Zhu, San Diego, CA (US); Derek Lorin Greenfield, Kensington, CA (US); Stephanie Rhianon Jones, Berkeley, CA (US); and Noah Charles Helman, El Cerrito, CA (US)
Assigned to INDUSTRIAL MICROBES, INC., Emeryville, CA (US)
Filed by Industrial Microbes, Inc., Alameda, CA (US)
Filed on Jan. 14, 2021, as Appl. No. 17/149,394.
Application 17/149,394 is a continuation of application No. 15/777,158, granted, now 10,894,951, previously published as PCT/US2016/062623, filed on Nov. 17, 2016.
Claims priority of provisional application 62/320,725, filed on Apr. 11, 2016.
Claims priority of provisional application 62/270,039, filed on Dec. 21, 2015.
Claims priority of provisional application 62/257,061, filed on Nov. 18, 2015.
Prior Publication US 2021/0254026 A1, Aug. 19, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/02 (2006.01); C12P 7/04 (2006.01); C12P 7/06 (2006.01); C12P 7/22 (2006.01)
CPC C12N 9/0073 (2013.01) [C12N 9/0071 (2013.01); C12P 7/04 (2013.01); C12P 7/06 (2013.01); C12P 7/22 (2013.01); C12Y 114/13025 (2013.01)] 5 Claims
 
1. A method for producing a chemical, comprising culturing a synthetic microorganism comprising at least one synthetic polynucleotide comprising at least one monooxygenase coding region encoding a diiron monooxegenase comprising SEQ ID NO: 10 and one protein folding chaperone encoding at least one protein folding chaperone, each linked to at least one promoter, under suitable culture conditions and for a sufficient period of time to produce the chemical.