	US 11,692,167 B2
	Chemically defined serum replacements for cell culture
Elizabeth Dale Corbin, Bozeman, MT (US)
Assigned to MONTANA STATE UNIVERSITY, Bozeman, MT (US)
Filed by Montana State University, Bozeman, MT (US)
Filed on Aug. 2, 2022, as Appl. No. 17/879,390.
Claims priority of provisional application 63/228,328, filed on Aug. 2, 2021.
Prior Publication US 2023/0052904 A1, Feb. 16, 2023
Int. Cl. C12N 5/00 (2006.01)

CPC C12N 5/0037 (2013.01) [C12N 2500/10 (2013.01); C12N 2500/32 (2013.01); C12N 2500/36 (2013.01); C12N 2500/38 (2013.01); C12N 2500/90 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01)]

18 Claims

1. A chemically defined media supplement comprising:

(a) Ascorbyl palmitate, docosahexanoic acid, palmitoleic acid, cholesterol sulfate and at least two components selected from the group consisting of ascorbic acid, silver, vitamin A, vitamin D3, and vitamin K; or

(b) ITSE (Insulin, transferrin, selenium, ethanolamine), Trace Elements A, Alpha Linolenic Acid, fibroblast growth factor, insulin-like growth factor, oleic acid, docosahexanoic acid, and vitamin D3; or

(c) ITSE (Insulin, transferrin, selenium, ethanolamine), Trace Elements A, Alpha Linolenic Acid, fibroblast growth factor, insulin-like growth factor, cholesterol sulfate and palmitoleic acid;

wherein the chemically defined media supplement is animal derived product free.