US 11,692,161 B2
Packed-bed bioreactor systems and methods of using the same
Ann Meejin Ferrie, Painted Post, NY (US); Vasiliy Nikolaevich Goral, Painted Post, NY (US); and Lori E Romeo, Elmira Heights, NY (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Filed by CORNING INCORPORATED, Corning, NY (US)
Filed on Feb. 4, 2020, as Appl. No. 16/781,807.
Claims priority of provisional application 62/910,696, filed on Oct. 4, 2019.
Claims priority of provisional application 62/801,325, filed on Feb. 5, 2019.
Prior Publication US 2020/0255783 A1, Aug. 13, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12M 1/34 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC C12M 25/14 (2013.01) [C12M 23/02 (2013.01); C12M 23/20 (2013.01); C12M 23/44 (2013.01); C12M 25/04 (2013.01); C12M 29/10 (2013.01); C12M 29/26 (2013.01); C12M 41/26 (2013.01); C12M 25/02 (2013.01); C12N 5/0068 (2013.01); C12N 5/0602 (2013.01); C12N 2513/00 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A bioreactor system comprising:
a cell culture vessel comprising a first end, a second end, and at least one reservoir between the first and second ends; and
a cell culture matrix disposed in the at least one reservoir, the cell culture matrix comprising a plurality of woven substrates each comprising a plurality of interwoven fibers with surfaces configured for adhering cells thereto,
wherein the bioreactor system is configured to flow material through the at least one reservoir in a flow direction from the first end to the second end,
wherein the substrates of the plurality of woven substrates are stacked such that each woven substrate is substantially parallel to each of the other woven substrates and is substantially parallel to the flow direction, and
wherein the plurality of woven substrates exhibits isotropic fluid flow permeability therethrough.