	US 11,692,159 B2
	Growth of bacterial host cells in gas permeable low-density polyethylene bags for production of plasmid DNA or recombinant molecules
Isabelle Louise Chambers, Fargo, ND (US)
Assigned to ALDEVRON, LLC, Fargo, ND (US)
Filed by aldevron, llc, Fargo, ND (US)
Filed on Jan. 11, 2021, as Appl. No. 17/146,023.
Application 17/146,023 is a continuation of application No. 16/816,187, filed on Mar. 11, 2020, granted, now 10,889,790.
Claims priority of provisional application 62/816,664, filed on Mar. 11, 2019.
Prior Publication US 2021/0139830 A1, May 13, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/70 (2006.01); C12P 21/00 (2006.01); C12M 1/04 (2006.01); C12M 1/00 (2006.01); G01N 1/28 (2006.01)

CPC C12M 23/14 (2013.01) [C12M 23/24 (2013.01); C12N 15/70 (2013.01); C12P 21/00 (2013.01); G01N 1/28 (2013.01)]

20 Claims

1. A method of growing bacterial host cells in a gas-permeable bag comprising the steps of:

providing a starter culture of bacterial host cells, wherein the starter culture comprises at least one colony of the host cells transformed with plasmids comprising a gene for a selection marker;

providing the gas-permeable bag;

adding media, a selection factor, and the starter culture host cells to the gas-permeable bag to form a main culture, wherein the selection factor is selected such that starter culture host cells expressing the gene for the selection marker are viable; and

incubating the gas-permeable bag containing the main culture such that the host cells grow to a desired concentration.