US 11,692,002 B2
RNA sequence adaptation
Stefan Heinz, Tübingen (DE); Tilmann Roos, Tübingen (DE); Dominik Vahrenhorst, Tübingen (DE); and Markus Conzelmann, Tübingen (DE)
Assigned to CureVac SE, Tübingen (DE)
Appl. No. 16/762,081
Filed by CureVac SE, Tübingen (DE)
PCT Filed Nov. 8, 2018, PCT No. PCT/EP2018/080692
§ 371(c)(1), (2) Date May 6, 2020,
PCT Pub. No. WO2019/092153, PCT Pub. Date May 16, 2019.
Claims priority of application No. PCT/EP2017/078647 (WO), filed on Nov. 8, 2017.
Prior Publication US 2022/0144877 A1, May 12, 2022
Int. Cl. C07H 1/06 (2006.01); A61K 31/713 (2006.01); C12Q 1/68 (2018.01)
CPC C07H 1/06 (2013.01) [A61K 31/713 (2013.01)] 22 Claims
 
1. A method for analysis or purification of a mixture comprising at least two harmonized RNA species, the method comprising:
a) obtaining the coding sequences for at least two RNA species, said at least two RNA species each having a length of 800 to 20,000 nucleotides wherein the sequence of at least one RNA species is adapted by altering the number of A and/or U nucleotides in the RNA sequence with respect to the number of A and/or U nucleotides in the original RNA sequence, said coding sequences of the at least two RNA species having a harmonized number of encoded A and U nucleotides that is no more than 50 different from each other;
b) synthesizing the at least two RNA species to produce at least two harmonized RNA species; and
c) analysing and/or purifying a mixture of said at least two harmonized RNA species by chromatography.