	US 11,691,817 B2
	Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol
Catherine Asleson Dundon, Minneapolis, MN (US); Pirkko Suominen, Maple Grove, MN (US); Aristos Aristidou, Excelsior, MN (US); Brian J. Rush, Minneapolis, MN (US); Kari Koivuranta, Helsinki (FI); Benjamin Matthew Hause, Currie, MN (US); Thomas William McMullin, Minnetonka, MN (US); and Kevin Roberg-Perez, Minneapolis, MN (US)
Assigned to CARGILL, INCORPORATED, Wayzata, MN (US)
Filed by CARGILL, INCORPORATED, Wayzata, MN (US)
Filed on Jan. 21, 2021, as Appl. No. 17/154,190.
Application 14/720,899 is a division of application No. 12/281,286, abandoned, previously published as PCT/US2007/006408, filed on Mar. 13, 2007.
Application 17/154,190 is a continuation of application No. 15/941,274, filed on Mar. 30, 2018, granted, now 10,899,544.
Application 15/941,274 is a continuation of application No. 14/720,899, filed on May 25, 2015, abandoned.
Claims priority of provisional application 60/781,674, filed on Mar. 13, 2006.
Prior Publication US 2021/0155411 A1, May 27, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/52 (2006.01); B65G 15/12 (2006.01); C12P 7/06 (2006.01); C12P 7/40 (2006.01); C12P 7/56 (2006.01); B65G 17/12 (2006.01); E01B 29/32 (2006.01)

CPC B65G 15/12 (2013.01) [B65G 17/12 (2013.01); C12N 15/52 (2013.01); C12P 7/06 (2013.01); C12P 7/40 (2013.01); C12P 7/56 (2013.01); E01B 29/32 (2013.01); Y02E 50/10 (2013.01); Y10T 137/85986 (2015.04)]

6 Claims

1. A yeast cell of species Issatchenkia orientalis that is genetically modified to produce lactate, said yeast cell further having a deletion or disruption of (i) a native gene encoding a glycerol-3-phosphate dehydrogenase and/or a native gene encoding a glycerol-3-phosphatase, and (ii) a pyruvate decarboxylase gene, wherein the yeast cell contains an exogenous lactate dehydrogenase gene.