US 11,691,146 B2
Flow cell with selective deposition or activation of nucleotides
Ali Agah, Menlo Park, CA (US); Aathavan Karunakaran, Berkeley, CA (US); Tarun Khurana, Fremont, CA (US); Stanley Hong, Palo Alto, CA (US); Merek Siu, Alameda, CA (US); Arvin Emadi, San Jose, CA (US); and Craig Ciesla, Mountain View, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Aug. 30, 2022, as Appl. No. 17/898,663.
Application 17/898,663 is a continuation of application No. 17/550,063, filed on Dec. 14, 2021, granted, now 11,453,003.
Application 17/550,063 is a continuation of application No. 17/254,469, granted, now 11,229,909, issued on Jan. 5, 2022, previously published as PCT/US2020/034510, filed on May 26, 2020.
Claims priority of provisional application 62/855,657, filed on May 31, 2019.
Prior Publication US 2022/0410154 A1, Dec. 29, 2022
Int. Cl. B01L 3/00 (2006.01); G11C 13/02 (2006.01); B01J 19/00 (2006.01); C12Q 1/6874 (2018.01); C12N 15/09 (2006.01)
CPC B01L 3/502715 (2013.01) [B01J 19/0046 (2013.01); B01L 3/502707 (2013.01); B01L 3/502738 (2013.01); G11C 13/02 (2013.01); B01J 2219/00711 (2013.01); B01J 2219/00713 (2013.01); B01J 2219/00722 (2013.01); B01L 2200/026 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0622 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/08 (2013.01); C12N 15/09 (2013.01); C12Q 1/6874 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An apparatus, comprising:
(a) a flow cell body defining one or more flow channels and a plurality of wells, each flow channel of the one or more flow channels to receive a flow of fluid, each well of the plurality of wells being fluidically coupled with the corresponding flow channel of the one or more flow channels, each well of the plurality of wells to contain at least one polynucleotide;
(b) a plurality of electrodes, each electrode of the plurality of electrodes being positioned in a corresponding well of the plurality of wells, the plurality of electrodes to effect writing of polynucleotides in the corresponding wells of the plurality of wells, each electrode of the plurality of electrodes defining an aperture, each electrode of the plurality of electrodes comprising a plurality of electrode segments arranged in quadrants, the aperture being defined at a central region of the arrangement of quadrants;
(c) an integrated circuit, the integrated circuit to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells of the plurality of wells to thereby generate polynucleotides representing machine-written data in the plurality of wells; and
(d) an imaging assembly to capture images indicative of one or more nucleotides in a polynucleotide.