CPC B01L 3/502715 (2013.01) [B01J 19/0046 (2013.01); B01L 3/502707 (2013.01); B01L 3/502738 (2013.01); G11C 13/02 (2013.01); B01J 2219/00711 (2013.01); B01J 2219/00713 (2013.01); B01J 2219/00722 (2013.01); B01L 2200/026 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0622 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/08 (2013.01); C12N 15/09 (2013.01); C12Q 1/6874 (2013.01)] | 20 Claims |
1. An apparatus, comprising:
(a) a flow cell body defining one or more flow channels and a plurality of wells, each flow channel of the one or more flow channels to receive a flow of fluid, each well of the plurality of wells being fluidically coupled with the corresponding flow channel of the one or more flow channels, each well of the plurality of wells to contain at least one polynucleotide;
(b) a plurality of electrodes, each electrode of the plurality of electrodes being positioned in a corresponding well of the plurality of wells, the plurality of electrodes to effect writing of polynucleotides in the corresponding wells of the plurality of wells, each electrode of the plurality of electrodes defining an aperture, each electrode of the plurality of electrodes comprising a plurality of electrode segments arranged in quadrants, the aperture being defined at a central region of the arrangement of quadrants;
(c) an integrated circuit, the integrated circuit to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells of the plurality of wells to thereby generate polynucleotides representing machine-written data in the plurality of wells; and
(d) an imaging assembly to capture images indicative of one or more nucleotides in a polynucleotide.
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