CPC B01J 19/0046 (2013.01) [C12N 15/1093 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00497 (2013.01); B01J 2219/00511 (2013.01); B01J 2219/00522 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00635 (2013.01); B01J 2219/00637 (2013.01); B01J 2219/00662 (2013.01); B01J 2219/00722 (2013.01); B01L 3/50857 (2013.01)] | 15 Claims |
1. A method for de novo oligonucleotide synthesis, comprising:
(a) providing predetermined sequences for a plurality of non-identical oligonucleotides;
(b) providing a device for synthesizing oligonucleotides, comprising:
(i) a solid substrate;
(ii) a main channel, wherein the main channel extends vertically into the solid substrate from an opening on a top side of the solid substrate; and
(iii) a plurality of microchannels, wherein each microchannel of the plurality of microchannels extends vertically from an opening on a bottom side of the solid substrate into the main channel, and wherein each microchannel of the plurality of microchannels comprises a fluid path having at least one turn of 45 degrees to 180 degrees in total, when viewed from a top view, and
wherein the device comprises more than 20,000 of the microchannels in total;
(c) adding a droplet of fluid comprising an extension reaction reagent specific to a microchannel;
(d) allowing sufficient time for an extension reaction step to occur; and
(e) repeating steps (c) and (d) until the plurality of non-identical oligonucleotides are synthesized.
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