CPC A61K 39/245 (2013.01) [A61K 35/17 (2013.01); A61P 35/00 (2018.01); C07K 14/03 (2013.01); C07K 14/7051 (2013.01); C07K 16/18 (2013.01); A61K 38/00 (2013.01); A61K 2039/5158 (2013.01)] | 10 Claims |
1. A method for forming a population of human T cells expressing a recombinant CAR protein and a T cell receptor specific for a CMV antigen, said method comprising: (1) administering a viral vector encoding: (a) a CMV pp65 protein and (b) a fusion protein comprising exon 4 of CMV protein IE1 (e4) and exon 5 of CMV protein IE2 (e5) to a CMV-seronegative human donor; (2) obtaining PBMCs from said human donor; (3) contacting said PBMCs with at least one CMV antigen; (3) allowing said contacted cells to produce a population of cells enriched for stimulated cells specific for CMV; (4) transducing at least a portion of said enriched population of cells with a vector expressing a recombinant CAR protein, thereby forming a population of human T cells expressing a T cell receptor specific for a CMV antigen and a recombinant CAR protein.
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