US 11,690,903 B2
Compositions for booster vaccination against dengue
Diana Coronel, Mexico City (MX); Betzana Zambrano, Montevideo (UY); Fernando Noriega, Cresco, PA (US); Tram Anh Wartel, Singapore (SG); and Yves Girerd-Chambaz, Paris (FR)
Assigned to SANOFI PASTEUR, Lyons (FR)
Appl. No. 16/652,902
Filed by Sanofi Pasteur, Lyons (FR)
PCT Filed Oct. 5, 2018, PCT No. PCT/IB2018/001219
§ 371(c)(1), (2) Date Apr. 1, 2020,
PCT Pub. No. WO2019/069130, PCT Pub. Date Apr. 11, 2019.
Claims priority of provisional application 62/568,525, filed on Oct. 5, 2017.
Prior Publication US 2020/0289637 A1, Sep. 17, 2020
Int. Cl. A61K 39/12 (2006.01); A61P 31/14 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/12 (2013.01) [A61P 31/14 (2018.01); A61K 2039/545 (2013.01); A61K 2039/70 (2013.01)] 22 Claims
 
1. A method of booster vaccination for inducing in a human subject a neutralizing antibody response against dengue virus, said method comprising administering to said human subject a composition comprising a dengue antigen of each of serotypes 1 to 4 wherein each of said dengue antigens is independently selected from the list consisting of: (a) a live attenuated dengue virus and (b) a live attenuated chimeric dengue virus,
wherein said subject has previously received a primary vaccination course against each of serotypes 1 to 4 of dengue virus, and said subject was dengue naïve before said primary vaccination course,
wherein said booster vaccination is administered at least one year after the end of the primary vaccination course and results in at least a 2-fold increase in the neutralizing antibody titre against each of serotypes 1 to 4,
wherein said dengue antigens of serotypes 1 to 4 comprise nucleic acid sequences having at least 90% identity to SEQ ID NOs: 1, 2, 3 and 4 respectively, and wherein
the amino acid at position 226 of the Envelope (E) protein of the dengue antigen of serotype 2 is threonine,
the amino acid at position 228 of the Envelope (E) protein of the dengue antigen of serotype 2 is glycine, and
the amino acid at position 251 of the Envelope (E) protein of the dengue antigen of serotype 2 is valine.