CPC A61K 35/50 (2013.01) [A61K 35/51 (2013.01); A61P 9/00 (2018.01); A61P 25/00 (2018.01); A61P 37/00 (2018.01); C12N 5/0665 (2013.01); C12N 5/0647 (2013.01); C12Y 304/24003 (2013.01)] | 12 Claims |
1. A composition comprising enhanced adherent stem cells that are isolated from a single source and cultured under a hypoxia condition and a pharmaceutically acceptable carrier,
wherein the single source is a mammalian umbilical cord, and
wherein the enhanced adherent stem cells have the following characteristics (a) to (f):
(a) having a high expression level of TAGLN, and having a high expression of one or more selected from the group consisting of STC1, LRRC17, and IL33, as compared with bone marrow stem cells;
(b) having a low expression level of ANXA4, TPMT, and NAKG, and having a low expression level of one or more selected from the group consisting of CCND1, SERPINE1, and PRNP, as compared with bone marrow stem cells;
(c) maintaining the morphology of adherent fibroblasts during subculture;
(d) having ability to differentiate into adipocytes, osteocytes, and chondrocytes;
(e) having one or more surface antigen characteristics selected from the group consisting of CD200+, Tra1-60−, CD3−, CD1a−, CD11c−, CD16−, CD86a−, CD8a−, CD40−, CD141+, CD61+, CD87+, and MIC A/B−; and
(f) having surface antigen characteristics of Oct4− and Nanog−.
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