CPC A61B 5/14865 (2013.01) [A61B 5/1459 (2013.01); A61B 5/14532 (2013.01); C12N 9/0004 (2013.01); C12N 11/02 (2013.01); C12N 11/14 (2013.01); G01N 27/3272 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/0301 (2013.01); C12Y 101/0302 (2013.01); C12Y 101/03003 (2013.01); C12Y 101/0303 (2013.01); C12Y 101/0304 (2013.01); C12Y 101/03005 (2013.01); C12Y 101/03007 (2013.01); C12Y 101/03008 (2013.01); C12Y 101/03011 (2013.01); C12Y 101/03012 (2013.01); C12Y 101/03015 (2013.01); C12Y 101/03016 (2013.01); C12Y 101/03018 (2013.01); C12Y 101/03019 (2013.01); C12Y 101/03023 (2013.01); C12Y 101/03027 (2013.01); C12Y 101/03029 (2013.01); C12Y 101/03037 (2013.01); C12Y 101/03038 (2013.01); C12Y 101/03041 (2013.01)] | 14 Claims |
1. A sensor, comprising:
a masking layer deposited onto a substrate comprising a plurality of electrodes, wherein the masking layer covers the plurality of electrodes;
an adhesion promoter deposited onto the substrate and onto the masking layer, wherein deposition of the adhesion promoter onto the plurality of electrodes is avoided due to the masking layer;
a signal transduction enzyme spin coated onto the substrate after the masking layer is removed from the substrate being coated with the adhesion promoter,
wherein the signal transduction enzyme is configured to detect an active compound capable of generating an electric current, and
wherein the signal transduction enzyme is configured so that a current measured by the plurality of electrodes settles within about 5% of its stable value within one second; and
a crosslinker deposited onto the signal transduction enzyme for crosslinking the signal transduction enzyme to produce a cross-linked enzyme film having a thickness of less than one micrometer.
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