	US 11,690,341 B2
	Cereal plants with improved cell wall properties
Søren Knudsen, Copenhagen V (DK); Sabrina Bodevin, Malmö (SE); Ole Olsen, Copenhagen S (DK); Hanne Thomsen, Copenhagen V (DK); Toni Wendt, Copenhagen V (DK); Jesper Harholt, Copenhagen V (DK); and Finn Lok, Copenhagen V (DK)
Assigned to Carlsberg A/S, Copenhagen V (DK)
Appl. No. 16/958,092
Filed by Carlsberg A/S, Copenhagen V (DK)
PCT Filed Dec. 21, 2018, PCT No. PCT/EP2018/086719 § 371(c)(1), (2) Date Jun. 25, 2020, PCT Pub. No. WO2019/129736, PCT Pub. Date Jul. 4, 2019.
Claims priority of application No. 17210954 (EP), filed on Dec. 28, 2017.
Prior Publication US 2021/0062208 A1, Mar. 4, 2021
Int. Cl. C12N 15/82 (2006.01); A01H 6/46 (2018.01); C12C 1/18 (2006.01); A01H 5/10 (2018.01); C12C 1/027 (2006.01); C12C 1/16 (2006.01); C12N 9/10 (2006.01)

CPC A01H 6/4624 (2018.05) [A01H 5/10 (2013.01); C12C 1/027 (2013.01); C12C 1/16 (2013.01); C12C 1/18 (2013.01); C12N 9/1051 (2013.01); C12N 15/8245 (2013.01); C12Y 204/01012 (2013.01)]

14 Claims

1. A barley plant or a part thereof, wherein the kernels of said barley plant have a reduced (1,3;1,4)-β-glucan content, and wherein said barley plant carries an induced mutation in the CslF6 gene, wherein said mutated CslF6 gene encodes a mutant CslF6 polypeptide comprising the amino acid sequence according to SEQ ID NO:1 or SEQ ID NO: 3 except that said mutant CslF6 comprises a substitution of amino acid 748 of SEQ ID NO:1 or SEQ ID NO:3 to a charged amino acid, wherein the charged amino acid is selected from the group consisting of Arg, His, Lys, Asp and Glu.