CPC G01N 33/54326 (2013.01) [B01D 21/0009 (2013.01); B03C 1/30 (2013.01); B03C 5/00 (2013.01); B03C 5/02 (2013.01); C12M 23/48 (2013.01); C12M 27/16 (2013.01); C12M 29/00 (2013.01); C12M 41/44 (2013.01); C12M 45/07 (2013.01); C12M 47/04 (2013.01); C12M 47/10 (2013.01); C12N 13/00 (2013.01); G01N 33/5436 (2013.01); B03C 1/01 (2013.01); B03C 1/288 (2013.01); B03C 2201/26 (2013.01); G01N 2333/31 (2013.01)] | 18 Claims |
1. A method for separating a biomolecule from a cell culture in a separation system, comprising the steps of:
providing a feed from a cell culture comprising said biomolecule to a magnetic separator and providing to the magnetic separator magnetic beads comprising ligands which bind said biomolecule;
separating by the magnetic separator said magnetic beads with the bound biomolecule from the rest of the feed;
forwarding said magnetic beads from the magnetic separator as a slurry with an added buffer to an elution cell, wherein the elution cell comprises an inlet in connection with an outlet from the magnetic separator, and a retaining arrangement for keeping the magnetic beads within the elution cell and allowing excess buffer to escape from the elution cell;
packing the slurry of said magnetic beads in the elution cell to provide a bed of magnetic beads within the elution cell with a void volume less than 60%; and
eluting the bound biomolecule in the elution cell.
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