US 11,686,726 B2
Method and system for separating biomolecules
Ola Lind, Uppsala (SE); Johan Fredrik Avallin, Uppsala (SE); Andreas Torbjorn Lundin, Uppsala (SE); Nils Norrman, Uppsala (SE); Ronnie Palmgren, Uppsala (SE); and Gustav Jose Rodrigo, Uppsala (SE)
Assigned to Cytiva Sweden AB, Uppsala (SE)
Appl. No. 16/470,607
Filed by GE Healthcare Bio-Sciences AB, Uppsala (SE)
PCT Filed Dec. 27, 2017, PCT No. PCT/EP2017/084620
§ 371(c)(1), (2) Date Jun. 18, 2019,
PCT Pub. No. WO2018/122246, PCT Pub. Date Jul. 5, 2018.
Claims priority of application No. 1622304 (GB), filed on Dec. 28, 2016; application No. 1622305 (GB), filed on Dec. 28, 2016; and application No. 1622307 (GB), filed on Dec. 28, 2016.
Prior Publication US 2020/0087614 A1, Mar. 19, 2020
Int. Cl. G01N 33/543 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12N 13/00 (2006.01); B01D 21/00 (2006.01); B03C 5/00 (2006.01); B03C 5/02 (2006.01); B03C 1/30 (2006.01); B03C 1/28 (2006.01); B03C 1/01 (2006.01)
CPC G01N 33/54326 (2013.01) [B01D 21/0009 (2013.01); B03C 1/30 (2013.01); B03C 5/00 (2013.01); B03C 5/02 (2013.01); C12M 23/48 (2013.01); C12M 27/16 (2013.01); C12M 29/00 (2013.01); C12M 41/44 (2013.01); C12M 45/07 (2013.01); C12M 47/04 (2013.01); C12M 47/10 (2013.01); C12N 13/00 (2013.01); G01N 33/5436 (2013.01); B03C 1/01 (2013.01); B03C 1/288 (2013.01); B03C 2201/26 (2013.01); G01N 2333/31 (2013.01)] 18 Claims
 
1. A method for separating a biomolecule from a cell culture in a separation system, comprising the steps of:
providing a feed from a cell culture comprising said biomolecule to a magnetic separator and providing to the magnetic separator magnetic beads comprising ligands which bind said biomolecule;
separating by the magnetic separator said magnetic beads with the bound biomolecule from the rest of the feed;
forwarding said magnetic beads from the magnetic separator as a slurry with an added buffer to an elution cell, wherein the elution cell comprises an inlet in connection with an outlet from the magnetic separator, and a retaining arrangement for keeping the magnetic beads within the elution cell and allowing excess buffer to escape from the elution cell;
packing the slurry of said magnetic beads in the elution cell to provide a bed of magnetic beads within the elution cell with a void volume less than 60%; and
eluting the bound biomolecule in the elution cell.