US 11,686,725 B2
Detection and treatment of neurological diseases
Yanhong Shi, Arcadia, CA (US); and Li Li, Glendale, CA (US)
Assigned to CITY OF HOPE, Duarte, CA (US)
Filed by CITY OF HOPE, Duarte, CA (US)
Filed on Jul. 8, 2019, as Appl. No. 16/505,534.
Claims priority of provisional application 62/713,475, filed on Aug. 1, 2018.
Prior Publication US 2020/0132674 A1, Apr. 30, 2020
Int. Cl. G01N 33/50 (2006.01); C12Q 1/6883 (2018.01); C07K 16/28 (2006.01); C12N 5/074 (2010.01); C12N 5/079 (2010.01); G16H 50/30 (2018.01); A61K 31/7105 (2006.01)
CPC G01N 33/5058 (2013.01) [C12N 5/0622 (2013.01); C12N 5/0696 (2013.01); C12Q 1/6883 (2013.01); G16H 50/30 (2018.01); A61K 31/7105 (2013.01); C07K 16/28 (2013.01); C12N 2310/141 (2013.01); C12N 2310/20 (2017.05); G01N 2570/00 (2013.01); G01N 2800/285 (2013.01); G01N 2800/2821 (2013.01); G01N 2800/2835 (2013.01)] 7 Claims
 
1. A method of determining if abnormal expression of a Chitinase-3-like 1 protein (CHI3L1) gene and/or a CHI3L1 receptor is associated with a neurological disease, comprising:
differentiating astrocytes from induced pluripotent stem cells (iPSCs) obtained from one or more healthy control subjects;
differentiating astrocytes from iPSCs obtained from a subject suffering from the neurological disease;
performing a transcriptome analysis of the astrocytes derived from the subject suffering from the neurological disease and a transcriptome analysis of the astrocytes derived from the one or more healthy control subjects;
comparing the results of both transcriptome analyses to determine if the CHI3L1 gene and/or the CHI3L1 receptor are substantially differentially expressed in the subject suffering from the neurological disease compared to the one or more healthy subjects; and if the CHI3L1 gene and/or a CHI3L1 receptor are differentially expressed; and
correcting the expression of the CHI3L1 gene and/or the CHI3L1 receptor,
wherein complete or partial restoration of one or more phenotypes of the neurological disease after correcting expression of the CHI3L1 gene and/or the CHI3L1 receptor indicates that the CHI3L1 gene and/or the CHI3L1 receptor are associated with the neurological disease.