US 11,686,684 B2
Raman spectroscopy based assay for both low and high abundant biomolecules in a biological fluid sample
David Fournier, Northborough, MA (US); and Rishikesh Pandey, Unionville, CT (US)
Assigned to CytoVeris, Inc., Farmington, CT (US)
Filed by CytoVeris Inc., Farmington, CT (US)
Filed on Oct. 28, 2021, as Appl. No. 17/513,824.
Claims priority of provisional application 63/106,534, filed on Oct. 28, 2020.
Prior Publication US 2022/0128477 A1, Apr. 28, 2022
Int. Cl. G01N 21/65 (2006.01); A61B 5/00 (2006.01); G01N 33/543 (2006.01); G01J 3/44 (2006.01)
CPC G01N 21/658 (2013.01) [A61B 5/0075 (2013.01); G01J 3/4412 (2013.01); G01N 33/54346 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method for assaying one or more high abundant biomolecules and one or more low abundant biomolecules within a biological fluid sample, comprising:
placing a biological fluid sample in contact with a first nanostructure surface;
interrogating the biological fluid sample with a light source, the biological fluid sample in contact with the first nanostructure surface, the interrogation using a surface enhanced Raman spectroscopic (SERS) technique configured to produce an enhanced Raman scattering from at least one of one or more high abundant biomolecules adsorbed on the first nanostructure surface;
detecting the enhanced Raman scattering from at least one of the one or more high abundant biomolecules using a light detector configured to produce first signals representative of the enhanced Raman scattering from the at least one of the one or more high abundant biomolecules;
placing the biological fluid sample in contact with at least one second nanostructure surface, the at least one second nanostructure surface functionalized with at least one targeting agent configured to target at least one or the one or more low abundant biomolecules;
interrogating the biological fluid sample with the light source, the biological fluid sample in contact with the at least one second nanostructure surface, the interrogation using the SERS technique configured to produce an enhanced Raman scattering from at least one of the one or more low abundant biomolecules captured by the at least one targeting agent functionalized on the at least one second nanostructure surface;
detecting the enhanced Raman scattering from the one or more low abundant biomolecules and producing second signals representative thereof;
assaying the biological fluid sample using the first signals and the second signals.