US 11,686,014 B2
Vector library for yeast two hybrid screening and method for identifying deubiquitinating enzyme binding to target protein using same
Kwang-Hyun Baek, Seoul (KR); So-Ra Kim, Gimcheon-si (KR); Seul-Ki Kwon, Seongnam-si (KR); Soo-Yeon Kim, Chuncheon-si (KR); and Da-Hye Lee, Seoul (KR)
Assigned to CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Pocheon-si (KR)
Appl. No. 16/636,523
Filed by CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Pocheon-si (KR)
PCT Filed Aug. 6, 2018, PCT No. PCT/KR2018/008876
§ 371(c)(1), (2) Date Feb. 4, 2020,
PCT Pub. No. WO2019/031781, PCT Pub. Date Feb. 14, 2019.
Claims priority of application No. 10-2017-0100255 (KR), filed on Aug. 8, 2017.
Prior Publication US 2021/0032619 A1, Feb. 4, 2021
Int. Cl. C40B 30/06 (2006.01); C12N 9/48 (2006.01); C12N 15/10 (2006.01); C12N 15/81 (2006.01); C12Q 1/37 (2006.01); C40B 40/02 (2006.01); C40B 40/08 (2006.01)
CPC C40B 30/06 (2013.01) [C12N 9/48 (2013.01); C12N 15/1055 (2013.01); C12N 15/81 (2013.01); C12Q 1/37 (2013.01); C40B 40/02 (2013.01); C40B 40/08 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A method for screening an agent having anti-cancer activity, the method comprising:
(i) treating with candidate materials a cell overexpressing a deubiquitinating enzyme USP1, USP7, USP12, or USP49 and Bax protein, followed by culturing the cell; and
(ii) measuring apoptosis of the cell cultured in Step (i) and selecting a material inducing apoptosis of the cell.