CPC C12P 7/6463 (2013.01) [C12R 2001/72 (2021.05)] | 13 Claims |
1. A method for producing cis-unsaturated fatty acid by recombinant Candida rugosa lipase 1 (rCRL1), the method comprising following operations:
(i) providing an oil-water mixture, wherein the oil-water mixture comprises 1 to 2 parts by weight of oil and 1 part by weight of water;
(ii) adding 0.02 to 0.25 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) into the oil-water mixture;
(iii) after operation (ii), emulsifying the oil-water mixture without the addition of organic solvents;
(iv) after operation (iii), hydrolyzing the emulsified oil-water mixture to generate the cis-unsaturated fatty acid;
(v) after operation (iv), performing an oil-water separation at a temperature of 55° C. to 65° C. and extracting an oil phase layer; and
(vi) after operation (v), performing a cooling and filtering step to obtain the cis-unsaturated fatty acid.
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