US 11,685,939 B2
Method for producing cis-unsaturated fatty acid by recombinant Candida rugosa lipase 1 (rCRL1)
Guan-Chiun Lee, Taipei (TW); Ting-Chun Kuo, New Taipei (TW); Shun-Niang Chen, Yilan County (TW); and Chia-Chi Lin, Hsinchu (TW)
Assigned to CHANT OIL CO., LTD., New Taipei (TW); and National Taiwan Normal University, Taipei (TW)
Filed by CHANT OIL CO., LTD., New Taipei (TW); and National Taiwan Normal University, Taipei (TW)
Filed on Apr. 14, 2021, as Appl. No. 17/230,155.
Claims priority of application No. 109134294 (TW), filed on Sep. 30, 2020.
Prior Publication US 2022/0098628 A1, Mar. 31, 2022
Int. Cl. C12P 7/6418 (2022.01); C12N 9/20 (2006.01); C12P 7/6409 (2022.01); C12P 7/6463 (2022.01); C12R 1/72 (2006.01)
CPC C12P 7/6463 (2013.01) [C12R 2001/72 (2021.05)] 13 Claims
 
1. A method for producing cis-unsaturated fatty acid by recombinant Candida rugosa lipase 1 (rCRL1), the method comprising following operations:
(i) providing an oil-water mixture, wherein the oil-water mixture comprises 1 to 2 parts by weight of oil and 1 part by weight of water;
(ii) adding 0.02 to 0.25 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) into the oil-water mixture;
(iii) after operation (ii), emulsifying the oil-water mixture without the addition of organic solvents;
(iv) after operation (iii), hydrolyzing the emulsified oil-water mixture to generate the cis-unsaturated fatty acid;
(v) after operation (iv), performing an oil-water separation at a temperature of 55° C. to 65° C. and extracting an oil phase layer; and
(vi) after operation (v), performing a cooling and filtering step to obtain the cis-unsaturated fatty acid.