CPC C12P 5/007 (2013.01) [C12N 9/1025 (2013.01); C12N 9/88 (2013.01); C12N 15/52 (2013.01); C12P 7/42 (2013.01)] | 35 Claims |
1. Recombinant cells capable of producing an isoprenoid precursor, wherein the cells:
(i) (a) have been modified such that the activity of a YfiQ polypeptide is modulated by decreasing, attenuating, or deleting the expression of a nucleic acid encoding the YfiQ polypeptide as compared to cells that have not been modified for such modulation, and
(b) comprise a heterologous nucleic acid encoding a CobB polypeptide, wherein the cells have been modified such that the activity of the CobB polypeptide is modulated by increasing the expression of the nucleic acid encoding the CobB polypeptide as compared to cells that have not been modified for such modulation; and
(ii) comprise two or more heterologous nucleic acids encoding two or more polypeptides of a mevalonate (MVA) pathway, wherein culturing of the recombinant cells in a suitable media provides for the production of the isoprenoid precursor; and
(iii) comprise a heterologous nucleic acid encoding a polyprenyl pyrophosphate synthase polypeptide, wherein culturing of the recombinant cells in a suitable media provides for production of the isoprenoid.
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