US 11,685,937 B2
Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-CoA-derived products
Marguerite A. Cervin, Redwood City, CA (US); Michael C. Miller, San Francisco, CA (US); Derek H. Wells, Palo Alto, CA (US); Alan J. Wolfe, Buffalo Grove, IL (US); and Madhukar S. Dasika, Ellicott City, MD (US)
Assigned to Danisco US Inc., Palo Alto, CA (US)
Filed by Danisco US Inc., Palo Alto, CA (US); and The Goodyear Tire & Rubber Company, Akron, OH (US)
Filed on May 11, 2020, as Appl. No. 16/872,329.
Application 16/872,329 is a continuation of application No. 15/120,037, granted, now 10,648,004, previously published as PCT/US2015/016954, filed on Feb. 20, 2015.
Claims priority of provisional application 61/942,546, filed on Feb. 20, 2014.
Prior Publication US 2020/0340017 A1, Oct. 29, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 5/00 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 15/52 (2006.01); C12P 7/42 (2006.01)
CPC C12P 5/007 (2013.01) [C12N 9/1025 (2013.01); C12N 9/88 (2013.01); C12N 15/52 (2013.01); C12P 7/42 (2013.01)] 35 Claims
 
1. Recombinant cells capable of producing an isoprenoid precursor, wherein the cells:
(i) (a) have been modified such that the activity of a YfiQ polypeptide is modulated by decreasing, attenuating, or deleting the expression of a nucleic acid encoding the YfiQ polypeptide as compared to cells that have not been modified for such modulation, and
(b) comprise a heterologous nucleic acid encoding a CobB polypeptide, wherein the cells have been modified such that the activity of the CobB polypeptide is modulated by increasing the expression of the nucleic acid encoding the CobB polypeptide as compared to cells that have not been modified for such modulation; and
(ii) comprise two or more heterologous nucleic acids encoding two or more polypeptides of a mevalonate (MVA) pathway, wherein culturing of the recombinant cells in a suitable media provides for the production of the isoprenoid precursor; and
(iii) comprise a heterologous nucleic acid encoding a polyprenyl pyrophosphate synthase polypeptide, wherein culturing of the recombinant cells in a suitable media provides for production of the isoprenoid.