CPC C12N 15/86 (2013.01) [C12N 15/01 (2013.01); C12N 15/63 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14152 (2013.01)] | 10 Claims |
1. An isolated host cell comprising a nucleic acid encoding Adeno-associated virus (AAV) Rep proteins Rep78 and Rep68 operably linked to an inducible promoter, wherein the internal AAV promoter p19 comprises a substitution at position 818 from a guanine to an adenine wherein the position 818 is numbered according to a complete AAV2 genome of GenBank Accession number AF043303, whereby the internal AAV promoter p19 is inactivated and the functionality of said Rep78 and Rep68 proteins is maintained.
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