US 11,685,931 B2
Application of a fragment of an isolated nucleotide sequence in construction of non-mineralized intermuscular bone of Danio rerio
Zexia Gao, Hubei (CN); Chunhong Nie, Hubei (CN); Shiming Wan, Hubei (CN); Yulong Chen, Hubei (CN); and Dejie Zhu, Hubei (CN)
Assigned to Hubei Hongshan Laboratory, Hubei (CN); and Huazhong Agricultural University, Hubei (CN)
Filed by Hubei Hongshan Laboratory, Hubei (CN); and Huazhong Agricultural University, Hubei (CN)
Filed on Dec. 17, 2021, as Appl. No. 17/554,881.
Claims priority of application No. 202011115842.3 (CN), filed on Oct. 19, 2020.
Prior Publication US 2022/0275394 A1, Sep. 1, 2022
Int. Cl. C12N 15/85 (2006.01); A01K 67/027 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)
CPC C12N 15/8509 (2013.01) [A01K 67/0275 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); A01K 2217/077 (2013.01); A01K 2227/40 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] 4 Claims
 
1. A method for constructing a zebrafish without intermuscular bones, comprising:
performing a mutation on a target sequence of SEQ ID NO:1 in the zebrafish, thereby obtaining a mutated sequence of SEQ ID NO: 2 in the zebrafish.