	US 11,685,927 B2
	Low glucosinolate pennycress meal and methods of making
Tim Ulmasov, Chesterfield, MO (US); John C. Sedbrook, Bloomington, IL (US); Michael David Marks, Roseville, MN (US); and Ratan Chopra, St. Paul, MN (US)
Assigned to COVERCRESS INC., St. Louis, MO (US); BOARD OF TRUSTEES OF ILLINOIS STATE UNIVERSITY, Normal, IL (US); and REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
Filed by COVERCRESS INC., St. Louis, MO (US); BOARD OF TRUSTEES OF ILLINOIS STATE UNIVERSITY, Normal, IL (US); and REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
Filed on Mar. 2, 2021, as Appl. No. 17/249,431.
Application 17/249,431 is a division of application No. 16/251,247, filed on Jan. 18, 2019, granted, now 10,988,772.
Claims priority of provisional application 62/619,360, filed on Jan. 19, 2018.
Prior Publication US 2021/0198683 A1, Jul. 1, 2021
Int. Cl. A01H 5/10 (2018.01); A01H 1/00 (2006.01); C12N 15/00 (2006.01); C12N 15/82 (2006.01); A23K 10/30 (2016.01); A01H 6/20 (2018.01); A23K 40/10 (2016.01); A23K 50/10 (2016.01)

CPC C12N 15/8245 (2013.01) [A01H 5/10 (2013.01); A01H 6/20 (2018.05); A23K 10/30 (2016.05); A23K 40/10 (2016.05); A23K 50/10 (2016.05)]

9 Claims

1. A pennycress seed comprising: (i) at least one loss-of-function mutation in an endogenous pennycress gene encoding the polypeptide of SEQ ID NO: 161 or an allelic variant thereof, wherein said loss-of-function mutation reduces expression of said polypeptide or reduces transcription factor activity of said polypeptide; or (ii) at least one transgene or genome rearrangement that suppresses expression of at least one endogenous pennycress gene that encodes the polypeptide of SEQ ID NO: 161 or an allelic variant thereof; wherein said allelic variants of SEQ ID NO: 161 have at least 95% sequence identity to SEQ ID NO: 161 and wherein said seed exhibits a reduction in sinigrin content in comparison to sinigrin content of a control seed which lacks said loss-of-function mutation, said transgene, or said genome rearrangement.