	US 11,685,923 B2
	Methods of enhancing translation ability of RNA molecules, treatments, and kits
Samie R. Jaffrey, New York, NY (US); and Kate D. Meyer, Durham, NC (US)
Assigned to CORNELL UNIVERSITY, Ithaca, NY (US)
Filed by CORNELL UNIVERSITY, Ithaca, NY (US)
Filed on Feb. 19, 2020, as Appl. No. 16/795,216.
Application 16/795,216 is a division of application No. 15/744,246, granted, now 10,584,343, previously published as PCT/US2016/042550, filed on Jul. 15, 2016.
Claims priority of provisional application 62/193,464, filed on Jul. 16, 2015.
Prior Publication US 2020/0199601 A1, Jun. 25, 2020
Int. Cl. C12N 15/67 (2006.01); C12P 21/02 (2006.01); C12P 19/34 (2006.01)

CPC C12N 15/67 (2013.01) [C12P 19/34 (2013.01); C12P 21/02 (2013.01); C12N 2310/333 (2013.01); C12N 2740/16043 (2013.01)]

16 Claims

1. An mRNA molecule comprising:

a heterologous 5′ untranslated region (UTR), wherein the 5′ untranslated region comprises an adenosine methylation motif, wherein the adenosine methylation motif is a nucleotide sequence DRACH, wherein D is A, G, or U; R is G or A; and H is A, C, or U;

a modified residue in the adenosine methylation motif of the 5′ untranslated region (UTR), wherein the modified residue is a methylated adenosine residue; and

a 5′ cap, wherein said mRNA molecule is a substrate of eIF4E-independent translation.