CPC C12N 15/102 (2013.01) [C12N 9/22 (2013.01); A61K 38/00 (2013.01); C07K 2319/71 (2013.01); C12Y 305/04004 (2013.01); C12Y 305/04005 (2013.01)] | 6 Claims |
1. An engineered, non-naturally occurring system comprising a catalytically inactive Cas13b effector protein (dCas13b) or a nucleotide sequence encoding the catalytically inactive Cas13b effector protein, wherein the truncated form of the Cas13b effector protein has been truncated at:
N-terminal Δ1-20, N-terminal Δ1-40, N-terminal Δ1-60, N-terminal Δ1-80, N-terminal Δ1-100, N-terminal Δ1-120, N-terminal Δ1-140, N-terminal Δ1-160, N-terminal Δ1-180, N-terminal Δ1-200, N-terminal Δ1-220, N-terminal Δ1-240, N-terminal Δ1-260, or N-terminal Δ1-300, wherein amino acid positions of the truncations correspond to amino acid positions of Prevotella sp. P5-125 Cas13b protein as set forth in SEQ ID NO: 82.
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