CPC C12N 5/0671 (2013.01) [C12N 5/0062 (2013.01); C12N 5/0068 (2013.01); G01N 33/5008 (2013.01); G01N 33/5091 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01); C12N 2533/70 (2013.01)] | 11 Claims |
1. A three-dimensional co-culture system of primary hepatocytes and liver non-parenchymal cells comprising:
a transwell comprising a membrane of polyvinyl alcohol (PVA) nanofibers, wherein the primary hepatocytes are attached to the membrane of PVA nanofibers; and
a culture plate comprising a support of polycaprolactone (PCL) nanofibers, wherein the liver non-parenchymal cells are attached to the support of PCL nanofibers,
wherein the transwell and the culture plate are stacked adjacently, and
wherein the transwell and the culture plate prevent a direct co-culture between the primary hepatocytes and the liver non-parenchymal cells and induce an indirect co- culture.
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