US 11,685,900 B2
Nanofiber-based long-term primary hepatocyte three-dimensional culture system and culturing method
Jong-Young Kwak, Suwon-si (KR); Min Gyu Song, Tongyeong-si (KR); So Hee Kim, Seoul (KR); and Min Ho Choi, Seoul (KR)
Assigned to NANOFAENTECH CO., LTD., Gimhae-si (KR)
Appl. No. 16/649,986
Filed by NANOFAENTECH CO., LTD., Gimhae-si (KR)
PCT Filed Sep. 21, 2018, PCT No. PCT/KR2018/011215
§ 371(c)(1), (2) Date Mar. 24, 2020,
PCT Pub. No. WO2019/059702, PCT Pub. Date Mar. 28, 2019.
Claims priority of application No. 10-2017-0123545 (KR), filed on Sep. 25, 2017; and application No. 10-2018-0110880 (KR), filed on Sep. 17, 2018.
Prior Publication US 2020/0239851 A1, Jul. 30, 2020
Int. Cl. C12N 5/071 (2010.01); C12N 5/00 (2006.01); G01N 33/50 (2006.01)
CPC C12N 5/0671 (2013.01) [C12N 5/0062 (2013.01); C12N 5/0068 (2013.01); G01N 33/5008 (2013.01); G01N 33/5091 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01); C12N 2533/70 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A three-dimensional co-culture system of primary hepatocytes and liver non-parenchymal cells comprising:
a transwell comprising a membrane of polyvinyl alcohol (PVA) nanofibers, wherein the primary hepatocytes are attached to the membrane of PVA nanofibers; and
a culture plate comprising a support of polycaprolactone (PCL) nanofibers, wherein the liver non-parenchymal cells are attached to the support of PCL nanofibers,
wherein the transwell and the culture plate are stacked adjacently, and
wherein the transwell and the culture plate prevent a direct co-culture between the primary hepatocytes and the liver non-parenchymal cells and induce an indirect co- culture.