CPC C12N 5/0655 (2013.01) [A61K 35/32 (2013.01); A61P 19/04 (2018.01); C12N 15/86 (2013.01); G01N 33/5044 (2013.01); C12N 2740/10043 (2013.01)] | 2 Claims |
1. A stable human chondroprogenitor cell line or stable human osteochondroprogenitor cell line that expresses at least five times less lubricin (PRG4) relative to chondrocytes derived from healthy adult tissue, wherein:
(a) the cell line expresses at least three times less matrilin 3 (MATN3) relative to chondrocytes derived from healthy adult tissue,
(b) the cell line expresses two times less type I collagen (COL1) relative to bone marrow-derived mesenchymal stem cells (BM-MSCs);
(c) the cell line expresses the mesenchymal cell surface markers CD29, CD49c, CD105, and CD166;
(d) the cell line does not express the bone marrow-derived mesenchymal stem cell surface marker SSEA4; and
(e) the cell line expresses the chondrocyte cell surface marker CD54.
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