US 11,685,889 B2
Detection of nuclease edited sequences in automated modules and instruments
Andrew Garst, Boulder, CO (US); Richard Fox, Boulder, CO (US); Eileen Spindler, Boulder, CO (US); Amy Hiddessen, Boulder, CO (US); Phillip Belgrader, Pleasanton, CA (US); Don Masquelier, Boulder, CO (US); Bruce Chabansky, Boulder, CO (US); and Michael Graige, Boulder, CO (US)
Assigned to INSCRIPTA, INC., Boulder, CO (US)
Filed by Inscripta, Inc., Boulder, CO (US)
Filed on Apr. 30, 2022, as Appl. No. 17/734,037.
Application 17/734,037 is a continuation of application No. 17/676,746, filed on Feb. 21, 2022, granted, now 11,365,383.
Application 17/676,746 is a continuation of application No. 17/401,362, filed on Aug. 13, 2021, granted, now 11,268,061, issued on Mar. 8, 2022.
Application 17/401,362 is a continuation of application No. 16/536,049, filed on Aug. 8, 2019, granted, now 11,142,740, issued on Oct. 12, 2021.
Claims priority of provisional application 62/841,213, filed on Apr. 30, 2019.
Claims priority of provisional application 62/781,112, filed on Dec. 18, 2018.
Claims priority of provisional application 62/779,119, filed on Dec. 13, 2018.
Claims priority of provisional application 62/769,805, filed on Nov. 20, 2018.
Claims priority of provisional application 62/735,365, filed on Sep. 24, 2018.
Claims priority of provisional application 62/718,449, filed on Aug. 14, 2018.
Prior Publication US 2022/0259543 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/00 (2006.01); C12M 3/00 (2006.01); C12M 1/42 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01); C12M 1/26 (2006.01)
CPC C12M 35/08 (2013.01) [C12M 23/44 (2013.01); C12M 33/14 (2013.01); C12N 15/11 (2013.01); C12N 15/902 (2013.01); C12N 2310/20 (2017.05)] 10 Claims
OG exemplary drawing
 
1. A cell editing system comprising:
a robotic handling instrument;
a growth component having a growth chamber to grow cells;
a transformation component having a transformation chamber for receiving the cells from the growth component and for receiving nucleic acids, to create transformed cells;
an editing component having a first chamber to receive the transformed cells, to substantially singulate the transformed cells into cell colonies;
a storage component; and
a processor operably coupled to the robotic handling instrument, the processor being configured to execute a script to:
transfer the cells from the growth component to the transformation component in response to a first signal from a first sensor;
transfer the nucleic acids to the transformation component in response to a second signal from a second sensor;
transfer the transformed cells to the editing component in response to a third signal from a third sensor; and
transfer a first cell colony, of the cell colonies, to the storage component in response to a fourth signal from a fourth sensor.