US 11,685,764 B2
Separation matrix
Gustav José Rodrigo, Uppsala (SE); Tomas Bjorkman, Uppsala (SE); Mats Ander, Uppsala (SE); and Jesper Ulf Hansson, Uppsala (SE)
Assigned to CYTIVA BIOPROCESS R&D AB, Uppsala (SE)
Filed by Cytiva BioProcess R&D AB, Uppsala (SE)
Filed on Apr. 2, 2021, as Appl. No. 17/221,438.
Application 17/221,438 is a continuation of application No. 16/893,574, filed on Jun. 5, 2020, granted, now 10,995,113.
Application 16/893,574 is a continuation of application No. 16/682,855, filed on Nov. 13, 2019, granted, now 10,711,035, issued on Jul. 14, 2020.
Application 16/682,855 is a continuation of application No. 16/096,952, granted, now 10,513,537, issued on Dec. 24, 2019, previously published as PCT/EP2017/061164, filed on May 10, 2017.
Application 16/096,952 is a continuation of application No. 15/282,367, filed on Sep. 30, 2016, granted, now 10,654,887.
Claims priority of application No. 1608229 (GB), filed on May 11, 2016; and application No. 1608232 (GB), filed on May 11, 2016.
Prior Publication US 2021/0221841 A1, Jul. 22, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A23J 1/00 (2006.01); C07K 1/22 (2006.01); B01D 15/38 (2006.01); B01J 20/24 (2006.01); B01J 20/28 (2006.01); B01J 20/286 (2006.01); B01J 20/32 (2006.01); C07K 14/31 (2006.01); C07K 16/00 (2006.01); C07K 16/06 (2006.01); C07K 17/10 (2006.01)
CPC C07K 1/22 (2013.01) [B01D 15/3809 (2013.01); B01J 20/24 (2013.01); B01J 20/286 (2013.01); B01J 20/28016 (2013.01); B01J 20/28078 (2013.01); B01J 20/3212 (2013.01); B01J 20/3274 (2013.01); C07K 14/31 (2013.01); C07K 16/00 (2013.01); C07K 16/065 (2013.01); C07K 17/10 (2013.01)] 40 Claims
 
1. A separation matrix comprising at least 15 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein:
a) said ligands comprise multimers of alkali-stabilized Protein A domains,
b) said porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50, v) of from 56 micrometers to 70 micrometers and a dry solids weight of from 55 mg/ml to 80 mg/ml.