US 11,685,763 B2
Bone protein extraction without demineralization
Timothy Paul Cleland, Pflugerville, TX (US); and Deepak Vashishth, Glenmont, NY (US)
Assigned to Rensselaer Polytechnic Institute, Troy, NY (US)
Filed by Rensselaer Polytechnic Institute, Troy, NY (US)
Filed on Mar. 1, 2019, as Appl. No. 16/289,782.
Application 16/289,782 is a division of application No. 15/533,652, previously published as PCT/US2015/065239, filed on Dec. 11, 2015.
Claims priority of provisional application 62/090,612, filed on Dec. 11, 2014.
Prior Publication US 2019/0194247 A1, Jun. 27, 2019
Int. Cl. C07K 1/14 (2006.01); A61K 35/32 (2015.01)
CPC C07K 1/145 (2013.01) [A61K 35/32 (2013.01)] 4 Claims
 
1. A bone protein extraction buffer effective to solubilize matrix proteins from a sample of solid bone, comprising: ammonium phosphate dibasic, ammonium bicarbonate, guanidine HCl, and at least one protease inhibitor, wherein the at least one protease inhibitor is selected from the group consisting of: sodium fluoride, sodium orthovanadate, sodium pyrophosphate, beta-glycerophosphate, and mixtures thereof,
wherein the bone protein extraction buffer does not include a demineralization agent, and the extraction buffer includes ammonium phosphate dibasic at a concentration of 400 mM.