CPC C07K 1/145 (2013.01) [A61K 35/32 (2013.01)] | 4 Claims |
1. A bone protein extraction buffer effective to solubilize matrix proteins from a sample of solid bone, comprising: ammonium phosphate dibasic, ammonium bicarbonate, guanidine HCl, and at least one protease inhibitor, wherein the at least one protease inhibitor is selected from the group consisting of: sodium fluoride, sodium orthovanadate, sodium pyrophosphate, beta-glycerophosphate, and mixtures thereof,
wherein the bone protein extraction buffer does not include a demineralization agent, and the extraction buffer includes ammonium phosphate dibasic at a concentration of 400 mM.
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