US 11,684,916 B2
Chip for polymerase chain reaction, method of operation chip, and reaction device
Wenliang Yao, Beijing (CN); Nan Zhao, Beijing (CN); Haochen Cui, Beijing (CN); Peizhi Cai, Beijing (CN); Fengchun Pang, Beijing (CN); Yue Geng, Beijing (CN); Le Gu, Beijing (CN); Yuelei Xiao, Beijing (CN); Hui Liao, Beijing (CN); Yingying Zhao, Beijing (CN); and Chuncheng Che, Beijing (CN)
Assigned to BEIJING BOE OPTOELECTRONICS TECHNOLOGY CO., LTD., Beijing (CN); and BOE TECHNOLOGY GROUP CO., LTD., Beijing (CN)
Appl. No. 16/632,934
Filed by BEIJING BOE OPTOELECTRONICS TECHNOLOGY CO., LTD., Beijing (CN); and BOE TECHNOLOGY GROUP CO., LTD., Beijing (CN)
PCT Filed Jan. 9, 2019, PCT No. PCT/CN2019/071016
§ 371(c)(1), (2) Date Jan. 22, 2020,
PCT Pub. No. WO2020/142938, PCT Pub. Date Jul. 16, 2020.
Prior Publication US 2021/0060556 A1, Mar. 4, 2021
Int. Cl. B01L 3/00 (2006.01); C12Q 1/686 (2018.01)
CPC B01L 3/502715 (2013.01) [C12Q 1/686 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/1827 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A reaction device, comprising a chip for polymerase chain reaction and a control unit, wherein the chip for polymerase chain reaction, comprising:
a sample adding region, a mixing region, and a temperature cycling region in a sequential arrangement wherein the sample adding region is configured to add a sample, for separating a sample liquid and a reaction system liquid which are stored in a liquid container into liquid drops, the mixing region is configured to allow the liquid drop from the sample liquid to be mixed with the liquid drop from the reaction system liquid, the temperature cycling region is configured to is configured to allow the liquid drop of the reaction liquid to perform an annealing-refolding-extending temperature cycling amplification process, so as to perform the amplification reaction; and
at least one driving unit group, wherein the at least one driving unit group comprises a plurality of driving units, and is configured to drive a liquid drop to move and sequentially pass through the sample adding region, the mixing region, and the temperature cycling region, the sample adding region, the mixing region and the temperature cycling region is arranged in a moving direction of the liquid drop, the plurality of driving unit comprising driving electrodes,
each of the at least one driving unit group comprises: a first driving unit sub-group, in the temperature cycling region and configured to drive the liquid drop to cyclically move in the temperature cycling region; and a second driving unit sub-group, in the mixing region and configured to drive the liquid drop to cyclically move in the mixing region, and the second driving unit sub-group comprises the driving units arranged in a ring to form a loop.