CPC A01K 67/0275 (2013.01) [A01K 67/027 (2013.01); C07K 14/43595 (2013.01); C12N 15/8509 (2013.01); G06Q 99/00 (2013.01); A01K 2217/05 (2013.01); A01K 2217/052 (2013.01); A01K 2217/206 (2013.01); A01K 2227/40 (2013.01); A01K 2267/0393 (2013.01); C12N 2830/008 (2013.01)] | 19 Claims |
1. A method of making a transgenic fluorescent fish progeny comprising the steps:
(a) selecting a first transgenic fluorescent fish comprising a first transgene encoding a first fluorescent protein, and a second transgene encoding a second fluorescent protein; and
(b) breeding the first transgenic fish with a second fish to make a transgenic fluorescent fish progeny, wherein the transgenic fluorescent fish progeny comprises in its genome:
(i) the first transgene encoding the first fluorescent protein, wherein the first transgene is under the control of a muscle specific promoter, and
(ii) the second transgene encoding the second fluorescent protein, wherein the second transgene is under the control of a ubiquitous fish promoter;
wherein the first and second transgene encode different fluorescent proteins, and wherein the transgenic fluorescent fish progeny visibly expresses a fluorescent color different from the color encoded by the first transgene and the second transgene, and wherein the transgenic fluorescent fish progeny exhibits a strong visible fluorescence over 75% to 100% of the body of said fish, optionally excluding fins and eyes, wherein at least one of the first and second transgene comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.
|