US 11,684,049 B2
Recombinant constructs and transgenic fluorescent ornamental fish therefrom
Alan Blake, Austin, TX (US); Richard Crockett, Wilton, CT (US); Jeffrey Essner, Ames, IA (US); Perry Hackett, Saint Paul, MN (US); and Aidas Nasevicius, Tampa, FL (US)
Assigned to GloFish LLC, Earth City, MO (US)
Filed by GloFish LLC, Earth City, MO (US)
Filed on Jun. 29, 2020, as Appl. No. 16/915,859.
Application 16/915,859 is a continuation of application No. 15/675,003, filed on Aug. 11, 2017, granted, now 10,743,526.
Application 15/675,003 is a continuation of application No. 15/041,253, filed on Feb. 11, 2016, granted, now 9,763,432, issued on Sep. 19, 2017.
Application 15/041,253 is a continuation of application No. 14/057,333, filed on Oct. 18, 2013, granted, now 9,271,478, issued on Mar. 1, 2016.
Application 14/057,333 is a continuation of application No. 13/663,210, filed on Oct. 29, 2012, granted, now 8,581,025, issued on Nov. 12, 2013.
Application 13/663,210 is a continuation of application No. 12/577,467, filed on Oct. 12, 2009, granted, now 9,295,237, issued on Mar. 29, 2016.
Application 12/577,467 is a continuation of application No. 11/839,364, filed on Aug. 15, 2007, granted, now 7,700,825, issued on Apr. 20, 2010.
Claims priority of provisional application 60/842,721, filed on Sep. 7, 2006.
Claims priority of provisional application 60/838,006, filed on Aug. 16, 2006.
Prior Publication US 2020/0323181 A1, Oct. 15, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2006.01); C07K 14/435 (2006.01); C12N 15/85 (2006.01); G06Q 99/00 (2006.01)
CPC A01K 67/0275 (2013.01) [A01K 67/027 (2013.01); C07K 14/43595 (2013.01); C12N 15/8509 (2013.01); G06Q 99/00 (2013.01); A01K 2217/05 (2013.01); A01K 2217/052 (2013.01); A01K 2217/206 (2013.01); A01K 2227/40 (2013.01); A01K 2267/0393 (2013.01); C12N 2830/008 (2013.01)] 19 Claims
 
1. A method of making a transgenic fluorescent fish progeny comprising the steps:
(a) selecting a first transgenic fluorescent fish comprising a first transgene encoding a first fluorescent protein, and a second transgene encoding a second fluorescent protein; and
(b) breeding the first transgenic fish with a second fish to make a transgenic fluorescent fish progeny, wherein the transgenic fluorescent fish progeny comprises in its genome:
(i) the first transgene encoding the first fluorescent protein, wherein the first transgene is under the control of a muscle specific promoter, and
(ii) the second transgene encoding the second fluorescent protein, wherein the second transgene is under the control of a ubiquitous fish promoter;
wherein the first and second transgene encode different fluorescent proteins, and wherein the transgenic fluorescent fish progeny visibly expresses a fluorescent color different from the color encoded by the first transgene and the second transgene, and wherein the transgenic fluorescent fish progeny exhibits a strong visible fluorescence over 75% to 100% of the body of said fish, optionally excluding fins and eyes, wherein at least one of the first and second transgene comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.