US 11,680,294 B2
Amplicon region as epigenetic marker for the identification of non-classical monocytes
Sven Olek, Berlin (DE)
Assigned to Precision for Medicine GmbH, Berlin (DE)
Appl. No. 16/758,992
Filed by Precision for Medicine GmbH, Berlin (DE)
PCT Filed Oct. 26, 2018, PCT No. PCT/EP2018/079406
§ 371(c)(1), (2) Date Apr. 24, 2020,
PCT Pub. No. WO2019/081707, PCT Pub. Date May 2, 2019.
Claims priority of application No. 102017125335.1 (DE), filed on Oct. 27, 2017.
Prior Publication US 2020/0340053 A1, Oct. 29, 2020
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6881 (2018.01)
CPC C12Q 1/6881 (2013.01) [C12Q 2600/154 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for producing and detecting an amplicon from bisulfite treated DNA comprising the mammalian genomic region of SEQ ID NO: 1 prior to bisulfite treatment, the method comprising
a) bisulfite treating isolated genomic DNA from a mammalian cell sample to generate bisulfite treated DNA, and
b) producing the amplicon by amplifying from the bisulfite treated DNA a region comprising the mammalian genomic region of SEQ ID NO: 1 prior to bisulfite treatment, wherein the amplifying is performed with a polymerase chain reaction (PCR), and
c) detecting the amplicon with a probe comprising the nucleic acid sequence of SEQ ID NO: 8,
wherein the amplicon comprises CA at CA positions 242 and 248 relative to SEQ ID NO: 2 and one or more CA at a CA position selected from positions 39, 89, 123, 169, and 206 relative to SEQ ID NO: 2.