CPC C12Q 1/6881 (2013.01) [C12Q 2600/154 (2013.01)] | 14 Claims |
1. A method for producing and detecting an amplicon from bisulfite treated DNA comprising the mammalian genomic region of SEQ ID NO: 1 prior to bisulfite treatment, the method comprising
a) bisulfite treating isolated genomic DNA from a mammalian cell sample to generate bisulfite treated DNA, and
b) producing the amplicon by amplifying from the bisulfite treated DNA a region comprising the mammalian genomic region of SEQ ID NO: 1 prior to bisulfite treatment, wherein the amplifying is performed with a polymerase chain reaction (PCR), and
c) detecting the amplicon with a probe comprising the nucleic acid sequence of SEQ ID NO: 8,
wherein the amplicon comprises CA at CA positions 242 and 248 relative to SEQ ID NO: 2 and one or more CA at a CA position selected from positions 39, 89, 123, 169, and 206 relative to SEQ ID NO: 2.
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