US 11,680,282 B2
Apparatus and method for distinguishing antibiotics susceptibility
Won Il Lee, Gyeonggi-do (KR); and Young Hyeon Park, Gyeonggi-do (KR)
Assigned to ACCUNOSE CO., LTD., Gyeonggi-Do (KR)
Filed by ACCUNOSE CO.,LTD., Gyeonggi-do (KR)
Filed on Jul. 28, 2021, as Appl. No. 17/386,687.
Claims priority of application No. 10-2020-0184547 (KR), filed on Dec. 28, 2020.
Prior Publication US 2022/0205013 A1, Jun. 30, 2022
Int. Cl. C12Q 1/00 (2006.01); C12Q 1/18 (2006.01); C12N 1/20 (2006.01); G01N 1/38 (2006.01); G01N 1/34 (2006.01); C12M 1/32 (2006.01); C12M 1/12 (2006.01); G01N 21/64 (2006.01)
CPC C12Q 1/18 (2013.01) [C12M 23/12 (2013.01); C12M 25/16 (2013.01); C12N 1/20 (2013.01); G01N 1/34 (2013.01); G01N 1/38 (2013.01); G01N 21/6428 (2013.01); C12N 2523/00 (2013.01); G01N 2021/6439 (2013.01)] 12 Claims
 
1. A method of testing an antibiotic susceptibility, the method comprising:
a cultivating step of dispensing and cultivating sample solution into culture wells including one or more comparative wells and a plurality of antibiotic wells receiving two or more kinds of antibiotics, respectively;
a preprocessing step of receiving the sample solution, which has been cultivated in the cultivating step, into a plurality of preprocessing wells each including magnetic particles and fluorescent particles that bond to one or more kinds of bacteria such that the bacteria and the magnetic particles and fluorescent particles bond to each other;
a planarizing step of receiving the sample solution, which has undergone the preprocessing step, into a plurality of image wells having magnetic members thereunder such that the magnetic particles bonding to the bacteria are arranged on the bottoms of the image wells;
a washing step of removing the sample solution from the image wells that have undergone the planarizing step;
an imaging step of taking fluorescent images of the image wells washed in the washing step; and
an analyzing step of determining an antibiotic tolerance/susceptibility of the sample solution by analyzing the fluorescent images taken in the imaging step.