	US 11,680,270 B2
	Recombinant acid-resistant yeast with inhibited lactate metabolism and alcohol production and method of producing lactic acid using the same
Jae Yeon Park, Daejeon (KR); Tae Young Lee, Daejeon (KR); and Ki Sung Lee, Daejeon (KR)
Assigned to SK Innovation Co., Ltd., Seoul (KR)
Filed by SK INNOVATION CO., LTD., Seoul (KR)
Filed on Oct. 7, 2020, as Appl. No. 17/64,817.
Claims priority of application No. 10-2019-0124701 (KR), filed on Oct. 8, 2019.
Prior Publication US 2022/0049262 A1, Feb. 17, 2022
Int. Cl. C12N 15/81 (2006.01); C12N 1/16 (2006.01); C12P 7/56 (2006.01)

CPC C12N 15/81 (2013.01) [C12N 1/16 (2013.01); C12P 7/56 (2013.01)]

3 Claims

1. A recombinant strain having lactic-acid-producing ability, in which a gene comprising the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2 encoding an enzyme converting lactate to pyruvate is deleted or attenuated from an acid-resistant yeast strain KCTC13508BP;

a gene encoding an alcohol dehydrogenase and/or a gene encoding a pyruvate decarboxylase is deleted; and

a gene comprising the nucleotide sequence of SEQ ID NO: 7 encoding a lactate dehydrogenase is introduced into the acid-resistant yeast strain.