	US 11,680,254 B2
	Engineered meganucleases specific for recognition sequences in the PCSK9 gene
Victor Bartsevich, Durham, NC (US); Derek Jantz, Durham, NC (US); James Jefferson Smith, Morrisville, NC (US); and Janel Lape, Wake Forest, NC (US)
Assigned to PRECISION BIOSCIENCES, INC., Durham, NC (US)
Appl. No. 16/606,856
Filed by Precision BioSciences, Inc., Durham, NC (US)
PCT Filed Apr. 20, 2018, PCT No. PCT/US2018/028607 § 371(c)(1), (2) Date Oct. 21, 2019, PCT Pub. No. WO2018/195449, PCT Pub. Date Oct. 25, 2018.
Claims priority of provisional application 62/516,966, filed on Jun. 8, 2017.
Claims priority of provisional application 62/488,403, filed on Apr. 21, 2017.
Prior Publication US 2020/0131489 A1, Apr. 30, 2020
Int. Cl. C12N 9/22 (2006.01); C12N 15/86 (2006.01); A61K 48/00 (2006.01)

CPC C12N 9/22 (2013.01) [C12N 15/86 (2013.01); A61K 48/00 (2013.01); C12N 2750/14143 (2013.01); C12N 2800/80 (2013.01)]

8 Claims

1. An engineered meganuclease that binds and cleaves a recognition sequence consisting of SEQ ID NO: 4 within a proprotein convertase subtilisin/kexin type 9 (PCSK9) gene, wherein said engineered meganuclease comprises the amino acid sequence of SEQ ID NO: 6.