US 11,679,387 B2
Cartridge for nucleic acid extraction and nucleic acid extraction method
Chin Sung Park, Seoul (KR); Jong Sun Kim, Seoul (KR); Jin Seok Kang, Seoul (KR); Jeong Jin Choi, Seoul (KR); and Jun Hong Min, Seoul (KR)
Assigned to SK Telecom Co., Ltd., Seoul (KR)
Filed by SK Telecom Co., Ltd., Seoul (KR)
Filed on Nov. 14, 2019, as Appl. No. 16/683,646.
Application 16/683,646 is a continuation of application No. PCT/KR2018/005428, filed on May 11, 2018.
Claims priority of application No. 10-2017-0060609 (KR), filed on May 16, 2017; application No. 10-2017-0060610 (KR), filed on May 16, 2017; and application No. 10-2017-0060611 (KR), filed on May 16, 2017.
Prior Publication US 2020/0078786 A1, Mar. 12, 2020
Int. Cl. B01L 3/00 (2006.01); G01N 1/28 (2006.01); G01N 1/34 (2006.01); B01L 7/00 (2006.01); C12Q 1/6844 (2018.01); G01N 21/64 (2006.01); C12N 13/00 (2006.01); C12N 15/10 (2006.01)
CPC B01L 3/502738 (2013.01) [B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 7/52 (2013.01); B01L 13/02 (2019.08); C12N 13/00 (2013.01); C12N 15/1013 (2013.01); C12Q 1/6844 (2013.01); G01N 1/286 (2013.01); G01N 1/34 (2013.01); G01N 21/6486 (2013.01); B01L 2200/10 (2013.01); B01L 2200/141 (2013.01); B01L 2300/041 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/123 (2013.01); B01L 2300/14 (2013.01); B01L 2300/1805 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/06 (2013.01); G01N 2001/2866 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A cartridge for nucleic acid extraction, comprising:
a chamber module comprising a plurality of chambers configured to extract nucleic acids from a sample, the plurality of chambers including a pretreatment chamber in which the sample is crushed and subject to homogenization, cell disruption, and purification in the pretreatment chamber;
an air valve module installed on the chamber module, the air valve module configured to control a pressure that moves a fluid between the plurality of chambers; and
a liquid valve module installed below the chamber module, the liquid valve module configured to move the fluid between the plurality of chambers,
wherein the pretreatment chamber comprises:
a chamber body including an internal space having an inlet and an outlet that is opposite the inlet, the sample placed into the internal space of the chamber body via the inlet;
a cup filter in the internal space of the chamber body of the pretreatment chamber, the cup filter between the inlet and the outlet of the internal space; and
a pretreatment component between the cup filter and the inlet of the of the internal space of the chamber body, the pretreatment component including a pretreatment fluid, a magnet block, and a plurality of cell disruption particles that are configured to crush the sample such that the nucleic acids flow out of cells of the sample into the pretreatment fluid,
wherein the cup filter is configured to filter the pretreatment fluid and pass a primarily purified liquid containing the nucleic acids out of the outlet of the internal space.