	US 11,679,180 B2
	Methods and materials for using fibrin supports for retinal pigment epithelium transplantation
Alan D. Marmorstein, Rochester, MN (US); Raymond Iezzi, Rochester, MN (US); Jarel K. Gandhi, Rochester, MN (US); and Jose S. Pulido, Rochester, MN (US)
Assigned to Mayo Foundation for Medical Education and Research, Rochester, MN (US)
Appl. No. 16/467,681
Filed by Mayo Foundation for Medical Education and Research, Rochester, MN (US)
PCT Filed Nov. 13, 2017, PCT No. PCT/US2017/061300 § 371(c)(1), (2) Date Jun. 7, 2019, PCT Pub. No. WO2018/106414, PCT Pub. Date Jun. 14, 2018.
Claims priority of provisional application 62/431,259, filed on Dec. 7, 2016.
Prior Publication US 2020/0061246 A1, Feb. 27, 2020
Int. Cl. A61L 27/38 (2006.01); C12N 5/079 (2010.01); A61K 35/36 (2015.01); A61K 35/30 (2015.01); A61K 38/36 (2006.01); A61K 38/48 (2006.01); A61L 27/22 (2006.01); A61L 27/52 (2006.01); A61L 27/34 (2006.01); A61L 27/36 (2006.01)

CPC A61L 27/3813 (2013.01) [A61K 35/30 (2013.01); A61K 35/36 (2013.01); A61K 38/363 (2013.01); A61K 38/484 (2013.01); A61L 27/225 (2013.01); A61L 27/34 (2013.01); A61L 27/3687 (2013.01); A61L 27/3808 (2013.01); A61L 27/3839 (2013.01); A61L 27/52 (2013.01); C12N 5/0621 (2013.01); C12Y 304/21007 (2013.01); A61L 2420/02 (2013.01); A61L 2430/16 (2013.01); C12N 2501/734 (2013.01); C12N 2501/999 (2013.01); C12N 2506/45 (2013.01); C12N 2533/56 (2013.01); C12N 2533/90 (2013.01)]

18 Claims

1. A retinal implant comprising (a) a retinal pigment epithelium monolayer having an apical surface and a basal surface and that is a flat, wrinkle-free monolayer, and (b) a fibrin hydrogel layer directly attached to said basal surface of said monolayer, wherein said apical surface is free of a fibrin hydrogel layer, wherein said retinal implant lacks patches of degraded fibrin hydrogel within said fibrin hydrogel layer, and wherein said fibrin hydrogel layer degrades when said retinal implant is implanted into an eye of a mammal.