CPC A01N 63/20 (2020.01) | 23 Claims |
1. A composition, comprising:
a) a plurality of non-intergeneric remodeled bacteria; and
b) at least one plant hormone selected from the group consisting of auxins, cytokinins, gibberellins, abscisic acid, ethylene, brassinosteroids, jasmonic acid, and strigolactones;
wherein the non-intergeneric remodeled bacteria produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour;
wherein the non-intergeneric remodeled bacteria have an average colonization ability per unit of plant root tissue of at least about 1.0×104 bacterial cells per gram of fresh weight of plant root tissue;
wherein a member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a gene selected from the group consisting of: nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof.
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