US 11,678,668 B2
Agricultural compositions comprising remodeled nitrogen fixing microbes
Mark Reisinger, Berkeley, CA (US); Ernest Sanders, Berkeley, CA (US); Richard Broglie, Berkeley, CA (US); and Karsten Temme, Berkeley, CA (US)
Assigned to PIVOT BIO, INC., Berkeley, CA (US)
Filed by Pivot Bio, Inc., Berkeley, CA (US)
Filed on Jan. 14, 2022, as Appl. No. 17/576,139.
Application 17/576,139 is a continuation of application No. 17/254,175, previously published as PCT/US2019/039217, filed on Jun. 26, 2019.
Claims priority of provisional application 62/808,693, filed on Feb. 21, 2019.
Claims priority of provisional application 62/690,621, filed on Jun. 27, 2018.
Prior Publication US 2022/0132861 A1, May 5, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01N 63/20 (2020.01)
CPC A01N 63/20 (2020.01) 23 Claims
 
1. A composition, comprising:
a) a plurality of non-intergeneric remodeled bacteria; and
b) at least one plant hormone selected from the group consisting of auxins, cytokinins, gibberellins, abscisic acid, ethylene, brassinosteroids, jasmonic acid, and strigolactones;
wherein the non-intergeneric remodeled bacteria produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour;
wherein the non-intergeneric remodeled bacteria have an average colonization ability per unit of plant root tissue of at least about 1.0×104 bacterial cells per gram of fresh weight of plant root tissue;
wherein a member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a gene selected from the group consisting of: nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof.